Effect of various pH levels on the sperm kinematic parameters of boars

The objective of this work was to identify the effects pH on structure kinematic sperm subpopulations using commercial doses boar semen diluted in Androstar Plus extender. We adjusted value samples 7.4, 7.6, 7.8, and 8.0, evaluated motility parameters an open-source computer-assisted analysis system. dataset individual for average values overall sperm. To these subpopulations, we reduced dimensionality through principal component followed by hierarchical clustering. then compared results across with corresponding control (i.e., basal value). found that increasing did not affect proportion motile but manner which they moved, rendering their movement more linear. progressive increased relative at all above 7.1, while low 7.8. distinguished three subpopulations. Subpopulation 1 7.8 being characterized velocity high linearity. 3, contrast, were relatively few showed curvilinear velocity. In study, demonstrated presence responded differentially changes pH.